ACTA Pharmaceutica Sciencia 2020 , Vol 58 , Num 2
Intralesional Microemulsions for Effective Dermal Delivery of Chondroitinase: Formulation, Characterisation and Evaluation
Landge Anil 1 Krishnamoorthy Kannan 1
1 Department of Pharmacy, Annamalai University, Annamalainagar- 608002, Tamilnadu, India
DOI : 10.23893/1307-2080.APS.05813 The rationale behind the microemulsion (ME) formulation was to achieve the efficacy of intralesional delivery and permeability of chondroitinase (CHASE) along with enhanced stability over conventional delivery mode and thus patient compliance. The formulation proceeded with the pseudo-ternary phase diagrams construction to identify microemulsions regions and to establish unique ratio of oil surfactants and aqueous phase. The components used for microemulsion formulation were oleic acid as oil phase, Tween 80 and propylene glycol as surfactantcosurfactant mixture and phosphate buffer pH 7.4 as aqueous phase. The concentration of CHASE in microemulsions was 2.96 U/mL which was stabilised with 1M trehalose solution (w/w). The physicochemical properties of microemulsion were determined. The localization of CHASE within the dermal membrane was determined by in vitro permeability study using excised porcine ear skin. The permeation and the penetration properties of CHASE loaded microemulsion were compared with stabilised aqueous solution of CHASE for varied duration. Keywords : Chondroitinase, intralesional, dermal delivery, in-vitro permeability

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