Editor-in-Chief Hatice Kübra Elçioğlu Vice Editors Levent Kabasakal Esra Tatar Online ISSN 2630-6344 Publisher Marmara University Frequency Bimonthly (Six issues / year) Abbreviation J.Res.Pharm. Former Name Marmara Pharmaceutical Journal
Marmara Pharmaceutical Journal 2011 , Vol 15 , Issue 1
Oxidative alterations during human platelet storage
Bahar Göker1, Derya Özsavcı1, Azize Şener1, Halil Aksoy1, Vedat Bağışgil1, Gülderen Yanıkkaya-Demirel2, Fikriye Uras1
1Marmara Üniversitesi, Eczacılık Fakültesi, Biyokimya AD, İstanbul, Türkiye
2Yeditepe Universitesi,Tıp Fakültesi, Tıbbi Mikrobiyoloji AD, İstanbul, Türkiye
DOI : 10.12991/201115443 During storage of platelet obtained by apheresis several changes occur. The aim of this study was to investigate the effect of storage on activation, apoptosis, protein pattern, lipid peroxidation, and the levels of nitric oxide (NO) and glutathione (GSH) of platelets. In this study, platelets obtained from healty donors (n=7) by apheresis were kept in an agitator for nine days at 20-24°C. The samples were taken on the 1st, 3 rd, 5 th and 9 th days and platelets were precipitated. Platelet activation with PAC-1 and CD62-P antibodies and platelet apoptosis were measured with Annexin-V using flow cytometer. Platelets were frozen and thawed four times and then NO, GSH and malondialdehyte (MDA) levels were assayed by spectrophotometry. Platelets protein pattern was investigated via sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) procedure. When compared to the 1st day, platelet CD62-P, PAC- 1 expressions and Annexin-V levels significantly increased on the 3rd, 5th and 9th days, while platelet NO and GSH levels significantly decreased on the 3rd, 5th and 9th days. Furthermore MDA levels significantly increased on only the 5th and 9th days. Mild changes occurred in the density of platelet protein bands. In conclusion, our results show that alterations of platelet activity during storage period may enhance platelet procoagulant activity which increases trombogenic risk. Therefore, for transfusion using fresh platelets or adjusting platelet preservation are strongly important for platelet behavior in vivo conditions. Keywords : Transfusion, platelet, activation, apoptosis, protein
Marmara University