Editor-in-Chief Hatice Kübra Elçioğlu Vice Editors Levent Kabasakal Esra Tatar Online ISSN 2630-6344 Publisher Marmara University Frequency Bimonthly (Six issues / year) Abbreviation J.Res.Pharm. Former Name Marmara Pharmaceutical Journal
Journal of Research in Pharmacy 2023 , Vol 27 , Issue 5
A novel fluorescent-based HPLC method for determination of urolithins
Emine Vildan BURGAZ1,Bahareh NOSHADI1,Hayrettin Ozan GULCAN1
1Department of Pharmaceutical Chemistry, Faculty of Pharmacy, Eastern Mediterranean University, Famagusta, TRNC, via Mersin 10 Turkey DOI : 10.29228/jrp.480 Urolithins are the bioavailable metabolites of ellagitannins, abundantly available in dietaries routinely consumed. The number of research studies growingly enlarges on the beneficial effects of these natural chemicals on human health. From this perspective, one aspect of scientific interest in urolithins covers the development of techniques for their qualitative and quantitative analysis in diverse samples. UV-based and tandem-mass spectroscopic analyses have been the major ones used and applied so far. However, the fluorescent characteristics of these molecules have not received sufficient attention. Within the scope of this research study, major urolithins (Urolithin A and B) concomitant to their methyl ether metabolites have been synthesized and their quantitative analysis has been investigated via an HPLC system connected to fluorescence detection. The results displayed the superior and comparable characteristics of the methodology developed against the previous techniques. Keywords : Urolithin A; Urolithin B; Urolithin methyl ethers; Fluorescence; HPLC; quantification
Marmara University