Editor-in-Chief
Hatice Kübra Elçioğlu
Vice Editors
Levent Kabasakal
Esra Tatar
Online ISSN
2630-6344
Publisher
Marmara University
Frequency
Bimonthly (Six issues / year)
Abbreviation
J.Res.Pharm.
Former Name
Marmara Pharmaceutical Journal
Journal of Research in Pharmacy
2023 , Vol 27 , Issue 5
9-Hexadecenoic Acid Rich HPLC Fraction of Pithecellobium dulce Methanolic Seed Extract Exhibits Potential Antiinflammatory Activity by Inhibiting IL-8, IL-6, and PGE2: Phytochemical Characterization, in-vitro and in-vivo Evaluation
1Department of Zoology, Faculty of Neurobiology, Osmania University, Hyderabad, India
DOI :
10.29228/jrp.458
Pithecellobium dulce (Roxb.) Benth. has been used as a folk medicine due to its wide range of
pharmacological applications. In light of the lack of significant studies examining the anti-inflammatory potential of
seeds and their phytochemical constituents, we investigated the molecular basis behind the therapeutic efficacy of
methanolic extract both in-vitro and in-vivo. The phytochemical investigation of methanolic seed extract using GC-MS
analysis revealed the presence of 30 molecules of diverse classes. Further solvent fractionation followed by RP-HPLC
separation demonstrated the presence of 9-Hexadecenoic acid and 8,11,14 (Z, Z, Z)-Eicosatrienoic acid and were further
structurally characterized by 1H-NMR and HRMS. 9-Hexadecenoic acid-rich HPLC fraction exhibited the highest %
inhibition of albumin denaturation and % reduction of IL-8 and IL-6 in HaCaT cells as well as NO and PGE-2 production
in RAW267.4 cells among other test samples. In-vivo anti-inflammatory findings revealed that 9-Hexadecenoic acidrich
fraction potentially reduced rat paw edema by 84.62% at 150 μg/mL, which is superior to the diclofenac. The results
of the present study revealed that the 9-hexadecenoic acid-rich fraction of methanolic seed extract of P. dulce displayed
potential anti-inflammatory activity both in-vitro and in-vivo, which suggests an efficient alternative in treating a
variety of ailments related to inflammation and pain.
Keywords :
Pithecellobium dulce; Methanolic seed extract; Anti-inflammatory activity; 9-hexadecenoic acid; GC-MS; HPLC