¹Department of Biochemistry, Vels Institute of Science, Technology and Advanced Studies, Pallavaram, Chennai - 600117, Tamilnadu, India
²Research lab, GIET School of Pharmacy, Rajahmundry-533296, Andhra Pradesh, India DOI : 10.29228/jrp.360 Green nanoparticle synthesis using biological systems, particularly plant extracts is a growing subject in nanotechnology. Leaves extract of Syringodium isoetifolium was used to successfully show the environmentally friendly manufacture of zinc oxide nanoparticles (ZnO NPs) and zinc nitrate were used as precursor in this study. The nanoparticles were examined using X-Ray Diffraction (XRD), Energy-Dispersive X-ray analysis (EDX), UV spectroscopy (UV-VIS), Scanning Electron Microscopy (SEM), and Fourier Transform Infrared Spectroscopy (FT-IR). The absorption peak in 223 nm range was discovered via UV–Vis spectroscopy. XRD and FTIR verified the involvement of Syringodium isoetifolium bioactive substances in the steadiness of zinc oxide nanoparticles. SEM and EDX investigation indicated an agglomerated asymmetrical, hexagonal morphology and presence of O, Zn, C and K. Elemental mapping study of produced ZnO NPs revealed 60% zinc distribution, 26% oxygen distribution, and 13% carbon distribution. The produced ZnO NPs had a mean particle size of 26 nm was determined using dynamic light scattering (DLS) measurements. Additionally, an in vitro assay was used to assess the ZnO NPs' cytotoxic effects on the HepG2 cell line. The ZnO NPs had a strong cytotoxic effect on the HepG2 cancer cell line, as shown by the results of the MTT assay. ZnO NPs exhibited excellent antimicrobial potencies against four different bacterial via pour plate method, and their zone of inhibition values were calculated. Keywords : Zinc oxide nanoparticles, Syringodium isoetifolium, Green synthesis, Characterization, MTT assay, Cytotoxicity potential