¹Department of Pharmaceutical Technology, Faculty of Pharmacy, Selçuk University, Konya, Türkiye
²Department of Pharmaceutical Technology, Faculty of Pharmacy, Hacettepe University, Ankara, Türkiye
³Department of Pharmaceutical Technology, Faculty of Pharmacy, İnönü University, Malatya, Türkiye
⁴Department of Pharmacology and Toxicology, Faculty of Veterinary, Ankara University, Ankara, Türkiye
⁵Department of Medical Parasitology, Faculty of Medicine, Kafkas University, Kars, Türkiye
⁶Department of Parasitology, Veterinary Control Central Research Institute Directorate, Ankara, Türkiye DOI : 10.29228/jrp.252 A simple, rapid and reproducible HPLC method has been developed and validated for the quantification of albendazole sulfoxide (ALBSOX) and praziquantel (PRZ), which can coexist in various dosage forms. Chromatographic separation was performed in gradient mode using a mobile phase consisting of an InertSustain® C18 column (150 × 4.6 mm, 5 μm) and acetonitrile: water (v/v) at a flow rate of 1.0 mL/min. The active substance peaks were well separated and detected by a DAD detector at 217 nm. The HPLC method was linear for ALBSOX and PRZ in the concentration range of 0.1-50 μg/mL. Limit of detection (LOD) was found to be 0.01 μg/mL for ALBSOX and 0.009 μg/mL for PRZ. The limit of quantification (LOQ) was found to be 0.03 μg/mL for ALBSOX and 0.027 μg/mL for PRZ. The developed HPLC method was validated based on ICH guidelines for specificity, linearity, system suitability, precision and accuracy. The method was applied for simultaneous quantification and characterization studies of ALBSOX and PRA in PLGA nanoparticles. Keywords : Albendazole sulfoxide; HPLC method; nanoparticle; praziquantel; validation