¹Department of Pharmaceutical Technology, Faculty of Pharmacy, Trakya University, Edirne, Turkey DOI : 10.29228/jrp.217 Most of the feminine hygiene products sold in the market contain heavy chemicals that damage the mucosa. The well-designed intim formulation cleans without damage, supports the flora, protects against dryness, and maintenances of balanced microflora. For this purpose, we designed formulations made with natural raw materials such as manuka oil and Salix alba bark extract and investigated their irritation potential and effectiveness. A healthy vagina is dominated by Lactobacillus that produces lactic acid, resulting in a characteristic acidic environment (pH 3– 4), as it correlates with vaginal health by inhibiting the growth of bacteria and may also play a part in local immune defense. This new manuka oil- salix bark extract containing wash formulation was specifically formulated to achieve a formulation pH of 4.6-4.8 to be compatible with the normal skin pH range and to help maintain vulvovaginal skin homeostasis and provide protection against harmful bacteria. The formulation we designed was used by volunteers for 4 weeks and was found suitable in terms of gentle clean, refreshing feeling, shooting the skin, smell and irritation properties. None of the volunteers have experienced any side effects during product application. Our study findings further support other studies that have found that women who use intim wash attitudes and perceptions about hygiene. Keywords : Feminine wash; manuka oil; salix bark extract; female intimate hygiene; vulvovaginal