Editor-in-Chief Hatice Kübra Elçioğlu Vice Editors Levent Kabasakal Esra Tatar Online ISSN 2630-6344 Publisher Marmara University Frequency Bimonthly (Six issues / year) Abbreviation J.Res.Pharm. Former Name Marmara Pharmaceutical Journal
Journal of Research in Pharmacy 2022 , Vol 26 , Issue 4
Glypican-3 as a potential hepatocellular carcinoma peptide vaccine candidate for the Indonesian population
Angelika1,Arli Aditya PARIKESIT2,Michael JONATHAN1,Nadya Marcelina JULIANTO1
1Department of Biomedicine, School of Life Sciences, Indonesia International Institute for Life Sciences, Jakarta 13210, Indonesia
2Department of Bioinformatics, School of Life Sciences, Indonesia International Institute for Life Sciences, Jakarta 13210, Indonesia
DOI : 10.29228/jrp.194 Hepatocellular carcinoma (HCC) is the third most common cancer-related death worldwide, including in Indonesia. However, to date, there is still no effective treatment. As immunotherapy has gained more attention, this study aims to develop a peptide vaccine derived from GPC3. NetCTLPan1.1 and NetMHCPan were utilized to discover GPC3 based CTL and HTL epitopes, respectively. The peptide sequence was analyzed for its immunogenicity, IFNγ inducing capabilities, and population coverage; six-strong peptide candidates were chosen to create the vaccine. The 3D structure of the peptide vaccine was predicted using RaptorX, refined using GalaxyWEB, and then evaluated for the Ramachandran favored score using SwissProt Expasy before being docked. A peptide vaccine of GPC3-derived peptide was constructed and proven to have potent immunogenicity, antigenicity, and population coverage. Docking results using the 3D structure against different HLA alleles reveal promising interactions, thus predicted to be able to induce T cell response. The peptide vaccine is highly potential as an alternative treatment for HCC, but further in vitro and in vivo studies should be performed to confirm these findings. Keywords : Hepatocellular carcinoma; vaccine; Indonesia; glypican-3; in silico
Marmara University