ACTA Pharmaceutica Sciencia 2019 , Vol 57 , Num 3
Isolation of Two Homologous Triterpenes with Antimalarial Activities from the Leaf Extract of Combretum Zenkeri
Wande Oluyemi 1-2 Babatunde Samuel 1 Kahlig Hans-Peter 3 Taramelli Donatella 4 Krenn Liselotte 2
1 University Of Ibadan, Pharmaceutical Chemistry Department, Ibadan/Oyo, Nigeria
2 University of Vienna, Department of Pharmacognosy, Althanstrasse, Vienna, Austria
3 University of Vienna, Department of Organic Chemistry, WahringerStrasse, Vienna, Austria
4 Universitàdegli Studi di Milano, Dipartimento di Scienze Farmacologiche, Milano, Milan, Italy
DOI : 10.23893/1307-2080.APS.05715 Combretum genus (Combretaceae) is used locally in the treatment of various diseases including malaria. Current study aimed to investigate the antiplasmodial activities of methanol extract of C. zenkeri in Plasmodium falciparum chloroquine sensitive (D10) and resistant (W2) strains and identify the bioactive principles. Repeated chromatographic separation was carried out on the chloroform fraction to afford the isolation of bioactive compounds which was characterized by application of spectroscopic techniques (ESI-MS, HR-ESIMS, 1D and 2D NMR). Antiplasmodial activities of chloroform fraction (D10; IC50= 12.57±1.57 µg/mL and W2; IC50= 12.14±0.95 µg/mL) showed more activity than the n-butanol fraction (D10; IC50= 61.98±3.25 µg/mL and W2; IC50= 61.26±8.64 µg/mL). Phytochemical investigation of the extract afforded isolation of two triterpenes with antimalarial activities. This paper identified for the first time the antimalarial principles in C. zenkeri as ursolic and oleanolic acids; which justified the local use of the plant in the treatment of malaria. Keywords : C. zenkeri, antimalarial, bioguided isolation, triterpenes, Plasmodium falciparum

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