Editor-in-Chief Hatice Kübra Elçioğlu Vice Editors Levent Kabasakal Esra Tatar Online ISSN 2630-6344 Publisher Marmara University Frequency Bimonthly (Six issues / year) Abbreviation J.Res.Pharm. Former Name Marmara Pharmaceutical Journal
Journal of Research in Pharmacy 2024 , Vol 28 , Issue 2
Quantification of alpha-lipoic acid in pharmaceutical samples using Pd(II) catalyzed ligand substitution reaction in SLS micellar medium
1Department of Chemistry, GLA University, Mathura, 281406, U.P., India
2Department of Chemistry, DDU Gorakhpur University, Gorakhpur, 273001, UP (India)
3Department of Chemistry, B. N College of Engineering & Technology, Lucknow, U.P., India
DOI : 10.29228/jrp.709 A novel, repeatable, and swift kinetic approach for determining alpha-lipoic acid (ALA) in sodium lauryl sulfate (SLS) micellar medium has been presented, and it has been connected to ALA determination in drug formulations. The approach is based on ALA inhibitory property. ALA (containing two sulfur atoms) forms a chelate with Pd2+, lowering the effective [Pd(II)], and ultimately, the Pd2+ catalyzed cyanide substitution rate from [Ru(CN)6]4- by pyrazine (Pz). Fixed times of 5 and 10 minutes were chosen under optimal reaction conditions with [Pyrazine] = 3.0 × 10-4 mole L-1, pH = 4.0 ± 0.02, Temp = 318 ± 0.2 K, I = 0.1 mole L-1 (NaClO4), [Ru(CN)64-] = 2.75 × 10-5 mole L-1, [Pd+2] = 6.0 × 10-5 mole L-1, and [SLS] = 8.5 × 10-3 mole L-1 to calculate the absorbance at 370 nm associated with the final substitution product [Ru(CN)5 Pz]3-. ALA's inhibiting influence on the Pd2+ catalyzed cyanide substitution with pyrazine from [Ru(CN)6]4-, has been represented by a modified mechanistic approach. The concentration of ALA in various water specimens can be measured at the micro-level down to 1.25 × 10-6 mole L-1 using the established kinetic spectrophotometric approach. The suggested method is highly reproducible and has been effectively applied to accurately quantify the ALA in pharmaceutical samples. Even as much as 1000 with [ALA], typical additives used in medications do not significantly hinder the determination of ALA. Keywords : Inhibitory effect; Alpha-lipoic acid; Surfactant medium; Pharmaceutical preparations; Pd(II); Excipients
Marmara University