Editor-in-Chief
Hatice Kübra Elçioğlu
Vice Editors
Levent Kabasakal
Esra Tatar
Online ISSN
2630-6344
Publisher
Marmara University
Frequency
Bimonthly (Six issues / year)
Abbreviation
J.Res.Pharm.
Former Name
Marmara Pharmaceutical Journal
Journal of Research in Pharmacy
2024 , Vol 28 , Issue 1
Validation of an UPLC method for the determination of Pioglitazone Hydrochloride active substance in Pharmaceutical Dosage Forms
1Institute of Health Sciences Communication, Marmara University, Istanbul, Turkey2Department of Analytical Chemistry, Faculty of Pharmacy, Marmara University, Basıbuyuk 34854 Istanbul, Turkey DOI : 10.29228/jrp.704 The present work reports an ultra-performance liquid chromatography (RP-UPLC) method for the quantitative determination of pioglitazone HCl (PGZ) in pharmaceutical dosage form. The chromatographic separation was performed on C18, BEH (50 mm x 2.1 mm, 1.7 μm) column using isocratic elution. The optimized mobile phase consists of phosphate buffer (pH:6) as a solvent-A and 55:45 v/v mixture of acetonitrile as solvent-B. Flow rate was 0.4 ml/min with UV detection at (λmax) 225 nm and the injection volume was set at 2 μL with retention time 1 min. The developed RP-UPLC method was validated as per International Conference on Harmonization (ICH) guidelines with respect to system suitability, specificity, precision, accuracy, linearity. Keywords : Diabetes; Pioglitazone hydrochloride; UPLC; Validation; Pharmaceutical Dosage Form