¹Department of Pharmacy, Poltekkes Kemenkes Bandung, Jl. Prof. Eyckman No. 24, Pasteur, Kecamatan Sukajadi, Kota Bandung, Jawa Barat, Indonesia, 40161
²Department of Pharmacy (Pharmaceutical Biology), Faculty of Mathematics and Science, Universitas Garut, Jl. Jati No. 42 B, Tarogong, Kabupaten Garut, Jawa Barat, Indonesia
³Department of Medical Laboratory Technology, Poltekkes Kemenkes Bandung, Jl. Babakan Loa No. 10 A – Gunung Batu, Pasir Kaliki, Kecamatan Cimahi Utara, Kota Cimahi, Jawa Barat, Indonesia, 40154 DOI : 10.29228/jrp.539 The body's immune system plays a very important role in defending the body from antigens and infectors that enter the body and strive to eliminate them. Along with increasing public awareness and knowledge, the development of medicines from herbal/natural ingredients is being intensively carried out. Ananas comosus is a type of plant that is widely spread in tropical countries including Indonesia. The fruit is rich in nutrients, vitamins, essential minerals, and the content of the enzyme bromelain which has a wide range of pharmacological activities, including its alleged efficacy as an immunomodulator.This study aims to determine the effective dose of squeezing the flesh and fruit stems of Ananas comosusas an immunomodulator against its phagocytic activity based on the carbon clearance method and the application of its immunomodulator in test animal models induced by Staphylococcus aureus bacteria. The experimental animals used were male mice of the BALB/c strain, which was divided into several groups, namely: normal control group, negative control, immunostimulant positive control(Stimuno Forte®), immunosuppressant positive control (methylprednisolone), Ananas comosus fruit flesh and stem squeezing test(ACFSF) dosis 1 (125 mg/kg bw), dose 2 (250 mg/kg bw), and dose 3 (500 mg/kg bw).Examination of these immunomodulatory activity tests, including: therate of carbon elimination velocity, the phagocytosis index, the organ index, and the percentage of phagocytic activity (%).The results of this study have shown that the ACFSF group with the most effective dose as an immunostimulant based on the carbon clearance method and the application of immunomodulators in test animal models induced by S. aureus bacteria is ACFSF dose 3 (500 mg/kg bw) with an index value phagocytos is 1,40 and the largest percentage of phagocytic activity (%) compared to the negative control group and close to the immunostimulant comparison group Stimuno Forte®. Keywords : Immunomodulatory; Ananas comosus; carbon clearance method; phagocytic index; Staphylococcus aureus-induced