Editor-in-Chief Hatice Kübra Elçioğlu Vice Editors Levent Kabasakal Esra Tatar Online ISSN 2630-6344 Publisher Marmara University Frequency Bimonthly (Six issues / year) Abbreviation J.Res.Pharm. Former Name Marmara Pharmaceutical Journal
Journal of Research in Pharmacy 2023 , Vol 27 , Issue 5
Topical anti-inflammatory and analgesic activities of Laportea decumana (Roxb) Wedd extract cream in rats
Djulfikri MEWAR1,Yulia Yusrini DJABIR2,Gemini ALAM3
1Graduate Program, Faculty of Pharmacy, Hasanuddin University, Makassar, Indonesia
2Laboratory of Clinical Pharmacy, Faculty of Pharmacy, Hasanuddin University, Makassar, Indonesia
3Laboratory of Phytochemistry, Faculty of Pharmacy, Hasanuddin University, Makassar, Indonesia
DOI : 10.29228/jrp.482 Laportea decumana is a plant that has traditionally been used to treat pain. This study aimed to determine the anti-inflammatory and analgesic activity of L. decumana leaf extract (LD extract) topical cream in rats. The antiinflammatory effects of LD cream at a concentration of 0.5%, 1%, and 2% extract were determined by carrageen-induced acute inflammation on rat hind paws. Hydrocortisone (1%) cream was used as a comparison. The volume of oedema and IL-6 level were analysed following one day of application. Another set of experiments was performed to assess the analgesic activity of LD cream by intraplantar injection of 1% formalin, using 30% methyl salicylate cream as a comparison. Pain indicators, including foot stamping and licking, were observed for 60 minutes. The results showed that rats received the 2% LD cream had reduced carrageenan-induced paw oedema of up to 13.1% at the 3rd hour, and 8.1% at the 4th hour, compared to the placebo (p < 0.05). Rats treated with the 2% LD cream also had the lowest level of IL-6 in their paw tissue, which was comparable to 1% hydrocortisone cream treatment. Similarly, in the analgesic tests, a significant decrease in the amount of foot stamping and licking was seen following the administration of 2% LD cream (p < 0.05). This analgesic effect was similar to that of 30% methyl salicylate cream. In conclusion, cream containing 2% LD extract provided anti-inflammatory and analgesic effects in rats, and might be useful for treating pain and inflammation in humans. Keywords : Laportea decumana; anti-inflammatory; Carrageenan; analgesic; formalin
Marmara University