Editor-in-Chief Hatice Kübra Elçioğlu Vice Editors Levent Kabasakal Esra Tatar Online ISSN 2630-6344 Publisher Marmara University Frequency Bimonthly (Six issues / year) Abbreviation J.Res.Pharm. Former Name Marmara Pharmaceutical Journal
Journal of Research in Pharmacy 2023 , Vol 27 , Issue 3
HPLC-DAD Screening and Antioxidant Activity of Phenolic Compounds of Salvia balansae de Noé Leaves Extract
Mohamed Malik MAHDJOUB1,Nourelimane BENZITOUNE1,Yacine MAIZ1,Nour el Houda AOUADI1,Mustapha Mounir BOUHENNA4,Nabil KADRI1
1Department of Biology, Faculty of Natural and Life Sciences and Earth Sciences, University of Bouira, 10000 Bouira, Algeria
2Department of Environmental Biological Sciences, Faculty of Natural and Life Sciences, Research Laboratory in Ecology and Environment (RLEE), University of Bejaia, 06000 Bejaia, Algeria
3Laboratory of Management and Valorization of Natural Resources and Quality Assurance (LGVRNAQ), Faculty of Natural and Life Sciences and Earth Sciences, University of Bouira, 10000 Bouira, Algeria
4Center for Scientific and Technical Research in Physical and Chemical Analysis (CRAPC), Bou-Ismail RP 42004 Bou Ismail, Tipaza, Algeria
5Laboratory of Biochemistry, Biophysics, Biomathematics, and Scientometry (L3BS), Faculty of Natural and Life Sciences, University of Bejaia, 06000 Bejaia, Algeria
DOI : 10.29228/jrp.400 Salvia balansae de Noé is known to be an important source of a wide variety of non-toxic bioactive compounds with lack of information hinders exploitation in food and pharmaceutical industries as natural antioxidants. In this work, the phenolic compounds from S. balansae leaves were extracted using an ultrasound-assisted methodology and were separated and identified by HPLC-DAD. The antioxidant activity was evaluated by ABTS, and DPPH scavenging assays, Iron (Fe2+) Chelating ability and reducing power (FRAP) tests. The total phenolic compounds (TPC) and total flavonoid content (TFC) were found with amounts of 49.63 ± 6.31 mg GAE/g DW and 8.14 ± 0.39 mg QE/g DW respectively. The ABTS, DPPH assays and metal chelating ability test demonstrated a higher antioxidant properties with IC50 values of 328.95 ± 5.29 μg/mL, 545.03 ± 3.27 μg/mL and 689.40 ± 0.86 μg/mL respectively. The reducing power at 700 nm increased considerably with concentration. In addition, the HPLC-DAD analysis showed that there were twelve detectable phenolic compounds and the benzoic acid, quercetin, myrecetin, hydroxyflavon, and ascorbic acid have been exclusively identified for the first time in S. balansae leaves extract and are rarely determined in other Salvia L. species. Therefore, these results provided new data about the main phenolic compounds present in S. balansae leaves extract with a strong antioxidant potential, which will be an alternative source for synthetic compounds. Keywords : Salvia balansae de Noé; phenolic compounds; antioxidant capacity; HPLC-DAD profile
Marmara University