Editor-in-Chief Hatice Kübra Elçioğlu Vice Editors Levent Kabasakal Esra Tatar Online ISSN 2630-6344 Publisher Marmara University Frequency Bimonthly (Six issues / year) Abbreviation J.Res.Pharm. Former Name Marmara Pharmaceutical Journal
Journal of Research in Pharmacy 2023 , Vol 27 , Issue 2
Antibacterial and antibiofilm activity of Melaleuca alternifolia (tea tree) essential oil against colistin resistant Salmonella enterica serotypes isolated from poultry environmental specimens
Merve Eylul KIYMACI1,Merve SAVLUK1,Mehmet GUMUSTAS2,Mehmet UVEY3,Nilgun UNAL1
1Department of Pharmaceutical Microbiology, Gülhane Faculty of Pharmacy, University of Health Sciences, Ankara, Turkey
2Department of Forensic Toxicology, Institute of Forensic Sciences, Ankara University, Ankara, Turkey
3Aviagen Anadolu AS, Veterinary Laboratory, Ankara, Turkey
DOI : 10.29228/jrp.333 The development of increasing resistance to antibiotics such as colistin, problems arise in the treatment of bacterial infections and make it necessary to search new alternative methods. For this purpose, plant-based approaches are among the important research topics depending on their traditional uses. The aim of the present study was to investigate the antibacterial and antibiofilm activity of tea tree oil purchased from a local market against a variety of 66 colistin resistant Salmonella enterica serotypes isolated from poultry farm environmental samples. Content analysis of TTO was determined by gas chromatography/mass spectroscopy. The antibacterial activity was determined by broth microdilution method, and antibiofilm activity was examined by crystal violet method. As a result, terpinen-4-ol was found as major component of TTO with 35.9% ratio. The MIC values of TTO were differed between 6250-12500 μg/mL. 27 of 66 isolates formed biofilm and 25 of 27 isolates belonged to S. Infantis. The biofilm reduction of TTO at subinhibitory concentration were found between 52-84.4%. Current study should be supported by future studies to determine the effectiveness of TTO to be among the agents that can be used together with antimicrobials in the attenuation of microorganisms. Keywords : antibacterial; biofilm; poultry; Salmonella enterica; tea tree oil; Melaleuca alternifolia; antibiotic resistance; colistin
Marmara University