Editor-in-Chief Hatice Kübra Elçioğlu Vice Editors Levent Kabasakal Esra Tatar Online ISSN 2630-6344 Publisher Marmara University Frequency Bimonthly (Six issues / year) Abbreviation J.Res.Pharm. Former Name Marmara Pharmaceutical Journal
Journal of Research in Pharmacy 2022 , Vol 26 , Issue 6
Quantification of N-acetylcysteine in drug formulations using inhibitory kinetic spectrophotometric method
Basant LAL1,Abhas ASTHANA2,Vinay Kumar SINGH3,Krishna SRIVASTAVA4
1Department of Chemistry, GLA University, Mathura- 281406, U.P., India
2Department of Chemistry, M.J.S. Government P.G. College, Bhind, M.P., India
3Department of Chemistry, Dr. Shakuntala Misra National Rehabilitation University, Lucknow, U.P., India
4Department of Chemistry, Shri Ramswaroop Memorial University, Barabanki, 222502, U.P., India
DOI : 10.29228/jrp.262 A simple, reproducible, and rapid kinetic method for the N-acetylcysteine (NAC) determination has been proposed and linked to NAC quantification in pharmaceutical preparations. The method is based on the inhibitory feature of N-acetylcysteine. NAC forms a stable complex with Hg2+ and reduces the actual Hg2+ concentration and ultimately the rate of reaction between pyrazine (Pz) and [Ru(CN)6]4- catalyzed by Hg2+. Under the optimized reaction conditions with [Ru(CN)64-] = 7.25 × 10-5 mole dm-3, I = 0.1 mole dm-3 (KCl), Temp = 45.0 ± 0.1 o C, [Hg+2] = 1.5 × 10-4 mole dm-3, [Pyrazine] = 8.5 × 10-4 mole dm-3, and pH = 4.0 ± 0.02, fixed time of 12 and 17 min was selected to compute the absorbance at 370 nm corresponding to the ultimate reaction product [Ru(CN)5 Pz]3-. The inhibitory action of NAC towards cyanide imitation by pyrazine from [Ru(CN)6]4-, catalyzed by Hg2+ has been demonstrated by a redesigned mechanistic scheme. With the proposed kinetic spectrophotometric method, the micro level quantification of NAC in distinct water samples can be done down to 1.5 × 10-6 mole dm-3. The developed procedure is highly reproducible and can be efficiently used to quantitatively estimate the NAC in the drug samples with high accuracy. The general additives present in drugs do not substantially interfere in the determination of NAC even up to 1000 times with [NAC]. Keywords : Hexacyanoruthenate(II); pharmaceutical preparations; excipients; catalyst inhibitor complex; Nacetylcysteine; ligand substitution reaction; inhibitory effect
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