¹Department of Pharmaceutical Chemistry, Faculty of Pharmacy, Erzincan Binali Yıldırım University, 24100 Erzincan, Turkey
²Department of Analytical Chemistry, Faculty of Pharmacy, Erzincan Binali Yıldırım University, 24100 Erzincan, Turkey
³Department of Biochemistry, Faculty of Pharmacy, Erzincan Binali Yıldırım University, 24100 Erzincan, Turkey
⁴Department of Pharmaceutical Chemistry, Faculty of Pharmacy, Cumhuriyet University, Faculty of Pharmacy, 58140 Sivas, Turkey
⁵Department of Pharmaceutical Chemistry, Faculty of Pharmacy, Gazi University, Ankara, 06330, Turkey DOI : 10.29228/jrp.239 Alzheimer's disease is a neurological disease characterized by the destruction of brain cells. In this disease, which causes a decrease in thought, memory and behavioral functions, the symptoms appear gradually with age. In this study, inhibition of acetylcholinesterase enzyme which is an important target in accordance with the cholinergic hypothesis, was studied. New 3(2H)pyridazinone-triazole derivatives were synthesized, confirmed by 1H-NMR, 13CNMR, HRMS analysis and their IC50 and Ki values were studied. The inhibition constants (Ki) of the compounds against the AChE enzyme ranged from 2.35 ± 0.18 to 5.15 ± 0.46 μM. The compound with the best inhibitory properties was compound 6d with a Ki value of 2.35 ± 0.18 μM. In addition, to support the experimental data, molecular docking studies were carried out with 6b, 6d, 6e and 6f compounds with AChE crystal structure (PDB ID:4M0E). Keywords : Acetylcholinesterase; alzheimer’s disease; molecular docking; pyridazinone; triazole