Editor-in-Chief İlkay Küçükgüzel Associate Editor Esra Tatar Online ISSN 2630-6344 Publisher Marmara University Frequency Bimonthly (Six issues / year) Abbreviation J.Res.Pharm. Former Name Marmara Pharmaceutical Journal
Marmara Pharmaceutical Journal 2016 , Vol 20 , Issue 3
Edaravone ameliorates valproate-induced gingival toxicity by reducing oxidative-stress, inflammation and tissue damage
Sehkar OKTAY1, Burcin ALEV1, Leyla KOC OZTURK1, Sevim TUNALI2, Sezin DEMIREL3, Ebru EMEKLI ALTURFAN1, Tugba TUNALI-AKBAY1, Serap AKYUZ3, Refiye YANARDAG2, Aysen YARAT1
1Department of Basic Medical Sciences, Faculty of Dentistry, MarmaraUniversity, 34854, Maltepe, Istanbul, Turkey
2Department of Chemistry, Faculty of Engineering, Istanbul University, 34320, Avcilar, Istanbul, Turkey
3Department of Pedodontics, Faculty of Dentistry, MarmaraUniversity, 34854, Maltepe, Istanbul, Turkey
DOI : 10.12991/mpj.20162096267 Valproic acid (2-n-propylpentanoic acid, VPA), the most widely used antiepileptic drug, has potential adverse effects and it can disrupt the oxidant and antioxidant balance. Edaravone (3-methyl-1-phenyl-2-pyrazoline-5-one, EDA) is a potent free radical scavenger. In this study, the effect of EDA on gingiva in VPA induced toxicity was investigated. Female Sprague Dawley rats were randomly divided into four groups: control group, EDA (30 mg/kg/day) given group, VPA (0.5 g/kg/day) given group, and VPA+EDA (in same dose and time) given group. EDA and VPA were given intraperitoneally for seven days. Total protein, lipid peroxidation (LPO), sialic acid (SA) and reduced glutathione (GSH) levels and catalase (CAT), glutathione-S-transferase (GST), glutathione peroxidase (GPx), superoxide dismutase (SOD), myeloperoxidase (MPO), alkaline phosphatase (ALP), acid phosphatase (ACP), sodium potassium ATPase (Na+/K+-ATPase) and tissue factor (TF) activities were determined in gingiva homogenates. The VPA-induced increases were statistically significant for MPO (p<0.01), ACP (p<0.01), Na+/K+-ATPase (p<0.05) and TF (p<0.01) activities, but not for LPO level and ALP activities. EDA treatment markedly blunted all such elevated anomalies. Conclusively, VPA induced oxidative and inflammatory gingival tissue damage, reactions that were appreciably reversed by concurrent administration of EDA. Keywords : Gingiva, valproic acid, edaravone, oxidative stress, inflammation
Marmara University