Editor-in-Chief İlkay Küçükgüzel Associate Editor Esra Tatar Online ISSN 2630-6344 Publisher Marmara University Frequency Bimonthly (Six issues / year) Abbreviation J.Res.Pharm. Former Name Marmara Pharmaceutical Journal
Marmara Pharmaceutical Journal 2016 , Vol 20 , Issue 2
Essential Oil Compositions and Antimicrobial Activity of Piper arborescens Roxb.
Wan Mohd Nuzul Hakimi Wan SALLEH1, Farediah AHMAD2, Heng Yen KHONG3
1Department of Chemistry, Faculty of Science, Universiti Teknologi Malaysia (UTM), 81310 Skudai, Johor, Malaysia
2Department of Chemistry, Faculty of Science, Universiti Teknologi Malaysia (UTM), 81310 Skudai, Johor, Malaysia
3School of Chemistry and Environment Studies, Faculty of Applied Sciences, Universiti Teknologi MARA (UiTM) Sarawak, Jalan Meranek, 94300 Kota Samarahan, Sarawak, Malaysia
DOI : 10.12991/mpj.20162071871 The essential oils obtained from the leaves and stems of Piper arborescens by hydrodistillation were analyzed by GC and GC–MS. Fifty components have been identified from the leaves and stems oils, comprise of thirty six (97.5%) and forty six (90.5%) components, respectively. The most abundant components in the leaves oil were β-phellandrene (24.3%), sabinene (16.3%), α-pinene (10.4%) and terpinen-4-ol (7.2%), while β-phellandrene (20.4%), methyl eugenol (11.0%) and β-caryophyllene (9.0%) were the main components in the stems oil. The essential oils were tested for antimicrobial activity by using disc diffusion and micro dilution methods for the minimum inhibitory concentration (MIC). The results showed that the leaves oil exhibited significant antimicrobial activity towards Staphylococcus aureus with MIC value 250 μg/ mL, while stems oil showed the activity against Gram positive bacteria, Pseudomonas aeruginosa and fungal, Aspergillus niger each with MIC value 500 μg/mL. Keywords : Essential oil, Piperaceae, Piper arborescens, antimicrobial activity
Marmara University