Editor-in-Chief İlkay Küçükgüzel Associate Editor Esra Tatar Online ISSN 2630-6344 Publisher Marmara University Frequency Bimonthly (Six issues / year) Abbreviation J.Res.Pharm. Former Name Marmara Pharmaceutical Journal
Marmara Pharmaceutical Journal 2015 , Vol 19 , Issue 1
The effect of oxytocin on the urinary bladder in male rats exposed to water avoidance stress: Light and electron microscopic investigation
Esin AK1, Esra CIKLER DULGER2, Ahmet Ozer SEHIRLI3, Sermin TETIK4, Rabia PISIRICILER1, Goksel SENER3, Sule CETINEL5
1Marmara Üniversitesi, Diş Hekimliği Fakültesi, Histoloji ve Embriyoloji BD, İstanbul, Türkiye
2Medipol Üniversitesi Tıp Fakültesi Histoloji ve Embriyoloji AbD, İstanbul, Türkiye
3Marmara Üniversitesi, Eczacılık Fakültesi Farmakoloji AbD, İstanbul, Türkiye
4Marmara Üniversitesi, Eczacılık Fakültesi, Biyokimya AbD, İstanbul, Türkiye
5Marmara Üniversitesi, Tıp Fakültesi, Histoloji ve Embriyoloji AbD, İstanbul, Türkiye
DOI : 10.12991/mpj.2015198606 We aimed to study the impact of water avoidance stress (WAS) on the bladder tissue of male rats and to reveal the effect of oxytocin on the morphologic and biochemical alterations related to stress induced interstitial cystitis .

Twenty four Wistar male rats were assigned into 4 groups and each included the same number of rats (n=6): 1.Control group (C): had no intervention, 2. Oxytocin group (OXY): recieved 5μg /kg oxytocin sc. for 5 days. 3. Chronic stress group (WAS): rats underwent 2 hours daily WAS for 5 days. 4. Chronic stress+Oxytocin group (WAS+OXY): recieved subcutaneous (sc) 5μg /kg oxytocin during chronic stress. Urinary bladder tissues were examined under light and electron microscopy. Oxidative stress was evaluated by malondialdehyde (MDA) and glutathione (GSH) levels. Moreover, systemic stress was measured with serum cortisol levels.

In light microscopic analysis of WAS group, desquamation of urothelium, edema in connective tissue, increased number of polymorphonuclear leukocytes and vascular congestion were detected while a prominent healing was observed in WAS+OT group. In electron microscopy, although there were degenerated urothelium, irregularity in glycosaminoglycon layer and a prominent dilatation in intercellular tight junctions in the WAS group, reversal of the injury was detected in the WAS+OT group. Serum levels of cortisole and tissue levels of MDA and GSH were found to be well correlated with the morphological findings.

Our study revealed that WAS induced interstitial cystitis in male rats causes similar morphologic and biochemical alterations in female rats. Additionally, oxytocin exerts a protective effect against this injury. Keywords : water avoidance stress, oxytocin, male rat, urinary bladder, histochemistry

Marmara University