Editor-in-Chief İlkay Küçükgüzel Associate Editor Esra Tatar Online ISSN 2630-6344 Publisher Marmara University Frequency Bimonthly (Six issues / year) Abbreviation J.Res.Pharm. Former Name Marmara Pharmaceutical Journal
Marmara Pharmaceutical Journal 2010 , Vol 14 , Issue 3
Quality control and anticholinesterase activity determinations on Sternbergia sicula
Derya Çiçek, Nehir Ünver Somer, Gülen İrem Kaya
Ege Üniversitesi Eczacılık Fak, Farmakognozi, İzmir, Türkiye DOI : 10.12991/201014446 Aerial and underground parts of Sternbergia sicula Tineo ex Guss., growing in Söke (Aydın), were collected during two different vegetation periods, namely flowering and fruting seasons. Quality control studies and anticholinesterase activity determinations were carried out on the prepared specimens. In the course of the quality control studies, assays for humidity, total ash, hydrochloric acid-insoluble ash and sulphated ash were performed on drug specimens prepared separately from plants collected in flowering and fruiting stages.The humidity, total ash, hydrochloric acid-insoluble ash and sulphated ash contents of the specimens ranged between 7.828-8.798 %, 7.086-16.924 %, 1.120-4.340 % and 11.102- 23.465 %, respectively. The total alkaloid contents of Sternbergia sicula specimens were determined by a titrimetric method. The amount of total alkaloids ranged between 0.122- 0.496 %. The highest content of total alkaloids was found in herba of S. sicula collected during flowering period. The lowest content of total alkaloids were detected in herba of S. sicula collected during fruiting period. Moreover, anticholinesterase activity of the total alkaloid extracts, prepared from drug specimens, were determined by Thin Layer Chromatography (TLC) assay, based on in vitro Ellman method. All of the alkaloidal extracts showed anticholinesterase activity. Keywords : Sternbergia sicula, Amaryllidaceae, Quantitative Determination, Anticholinesterase Activity
Marmara University