Editor-in-Chief İlkay Küçükgüzel Associate Editor Aslı Türe Online ISSN 2630-6344 Publisher Marmara University Frequency Bimonthly (Six issues / year) Abbreviation J.Res.Pharm. Former Name Marmara Pharmaceutical Journal
Journal of Research in Pharmacy 2021 , Vol 25 , Issue 4
Antioxidant, xanthine oxidase inhibitory and antibacterial activities of selected galactogogue Thai medicinal plant water and ethyl acetate extracts
Prapairat SEEPHONKAI1,Natwipha MONGKOLSIRI1,Wiranya THIABPHET1,Rattanaphorn TRAISATHIT1,Sutthira SEDLAK2,Komgrit WONGPAKAM2,Taweesak DHAMMARAJ3,Aphidech SANGDEE4
1Nanotechnology Research Unit, Department of Chemistry, Faculty of Science, Mahasarakham University, Khamriang, Kantarawichai, Maha Sarakham, 44150, Thailand and Center of Excellence for Innovation in Chemistry (PERCH-CIC)
2Walairukhavej Botanical Research Institute, Mahasarakham University, Khamriang, Kantarawichai, Maha Sarakham, 44150, Thailand
3Elderly Health Innovations from Thai Local Wisdom Research Unit, Faculty of Pharmacy, Mahasarakham University, Khamriang, Kantarawichai, Maha Sarakham, 44150, Thailand
4Microbiology and Applied Microbiology Research Unit, Department of Biology, Faculty of Science, Mahasarakham University, Khamriang, Kantarawichai, Maha Sarakham, 44150, Thailand
DOI : 10.29228/jrp.42 Water and ethyl acetate extracts of sixteen galactogogue Thai medicinal plants collected in Northeastern Thailand were evaluated for the antioxidant activity in terms of 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging property and total phenolic content (TPC), xanthine oxidase inhibitory and antibacterial activities. Among the extracts, both of the water and ethyl acetate extracts from the stem bark of Caesalpinia sappan (CS) and Ochna integerrima (OI) exhibited potent DPPH radical scavenging capacity (IC50 9.47 ± 0.59 µ 13.25 ± 0.52 µg/mL) as compared to ascorbic acid (IC50 5.39 ± 0.32 µg/mL) and high TPC (632.89 ± 10.18 - 985.34 ± 0.76 mg gallic acid equivalent (GAE)/g of extract). The strongest xanthine oxidase inhibitory property was detected in the ethyl acetate extract from the root of Clausena harmandiana (CH) followed by CS and OI extracts showing %inhibition of 99.22 ± 0.22, 78.00 ± 0.79 and 68.67 ± 1.14, respectively, at the tested concentration of 50 μg/mL and referenced to allopurinol. The water extracts of CS and OI also possessed good antibacterial activity against eight tested pathogenic bacteria especially Gram-positive, S. aureus (MSSA) DMST 2933, S. aureus (MRSA) DMST 20651 and Bacillus cereus ATCC 11778, with the MIC and MBC values ranging from 0.39 - 1.56 mg/mL. The antibacterial property of OI extract and xanthine oxidase inhibitory activity of CH and OI extracts were first reported. Keywords : Antioxidant activity; xanthine oxidase inhibition; antibacterial activity; galactogogue; DPPH; total phenolic content
Marmara University