Editor-in-Chief İlkay Küçükgüzel Associate Editor Aslı Türe Online ISSN 2630-6344 Publisher Marmara University Frequency Bimonthly (Six issues / year) Abbreviation J.Res.Pharm. Former Name Marmara Pharmaceutical Journal
Journal of Research in Pharmacy 2020 , Vol 24 , Issue 6
A comparative study of phenolic profiles and biological activities of Allium sphaerocephalon L. subsp. sphaerocephalon L. and Allium sphaerocephalon L. subsp. trachypus (Boiss. Et Spruner) K. Richter
Ahmet EMİR1,Ceren EMİR2
1Department of Pharmacognosy, Faculty of Pharmacy, Ege University, İzmir, Turkey
2Department of Pharmacognosy, Faculty of Pharmacy, Ege University, İzmir, Turkey
DOI : 10.35333/jrp.2020.248 Allium sphaerocephalon subsp. sphaerocephalon, the common name is known as “Round-Headed Leek”, is an edible plant in different countries. In the present study, bulb, stem and flower extracts of A. sphaerocephalon subsp. sphaerocephalon were compared in terms of phenolic profile and enzyme inhibitory activity, with A. sphaerocephalon subsp. trachypus which is not an edible species. Total phenolic and flavonoid contents of extracts were determined by spectrophotometrically, and bulb parts of both plants were found to be rich in phenolics. Twenty-four selected phenolic compounds were investigated qualitatively and quantitatively in the extracts by LC-MS-MS. Gallic acid (458.4-1246.4 μg/g extract) as phenolic acid and 3-O-methyl quercetin (46.6-136.2 μg/g extract) as a flavonoid were the major compounds in all extracts. In addition, enzyme (acetylcholinesterase (AChE), butyrylcholinesterase (BuChE) and tyrosinase) inhibitory potentials of all samples were performed using a 96-well microplate reader. Flower parts ( IC50, 11.49 μg/mL for AChE) and bulb parts of (IC50, 16.62 μg/mL for BuChE, 65.94 μg/mL for tyrosinase) A. sphaerocephalon subsp. sphaerocephalon were the most potent samples. Also, the results of all analyses were subjected to principal component analysis, to gain insight into the relationship between data of assays. Keywords : Allium sphaerocephalon; phenolics; cholinesterases; tyrosinase
Marmara University