Editor-in-Chief İlkay Küçükgüzel Associate Editor Aslı Türe Online ISSN 2630-6344 Publisher Marmara University Frequency Bimonthly (Six issues / year) Abbreviation J.Res.Pharm. Former Name Marmara Pharmaceutical Journal
Journal of Research in Pharmacy 2020 , Vol 24 , Issue 6
Isolation and characterization of chemical constituents from the mushroom Clitocybe nebularis
Morteza YAZDANI1,Zoltán BÉNI2,Miklós DÉKÁNY2,Viktor PAPP3,Andrea LÁZÁR5,Katalin BURIÁN5,Judit HOHMANN1,Attila VÁNYOLÓS1
1Department of Pharmacognosy, Interdisciplinary Excellence Centre, University of Szeged, H-6720 Szeged, Hungary
2Spectroscopic Research Department, Gedeon Richter Plc., Gyömrői út 19-21, H-1103 Budapest, Hungary
3Department of Botany, Szent István University, H-1118 Budapest, Villányi út 29-43, Hungary
4Interdisciplinary Centre of Natural Products, University of Szeged, H-6720 Szeged, Hungary
5Department of Clinical Microbiology, University of Szeged, H-6720 Szeged, Hungary
6Department of Pharmacognosy, Semmelweis University, H-1085 Budapest, Hungary
DOI : 10.35333/jrp.2020.250 In the course of our mycochemical studies the extract of Clitocybe nebularis was investigated with the aim to identify its bioactive secondary metabolites. Multistep chromatographic purification of the MeOH extract of C. nebularis resulted in the isolation of two steroids and an organic acid from the CHCl3 and ethyl acetate soluble fractions. The structures of the compounds were determined by NMR and MS spectroscopy as 5α-ergosta-7,22-diene-3β,5,6β-triol (cerevisterol) (1), (22E,24S)-5α-ergosta-7,22-diene-3β,5,6β,9α-tetraol (2), and indole-3-carboxylic acid (3). The antimicrobial activity of the compounds was analyzed by agar disc diffusion method against human pathogen strains of Streptococcus agalactiae, Staphylococcus epidermidis, Moraxella catarrhalis, Haemophilus influenzae, and Proteus mirabilis. The susceptibility assay revealed that compounds 2 and 3 have weak antimicrobial activity against M. catarrhalis. The current study represents the first isolation of compounds 1–3 from C. nebularis. Keywords : Clitocybe nebularis; steroids; antimicrobial activity; Tricholomataceae
Marmara University