Editor-in-Chief İlkay Küçükgüzel Associate Editor Aslı Türe Online ISSN 2630-6344 Publisher Marmara University Frequency Bimonthly (Six issues / year) Abbreviation J.Res.Pharm. Former Name Marmara Pharmaceutical Journal
Journal of Research in Pharmacy 2020 , Vol 24 , Issue 5
Spectrofluorimetric analysis of ticagrelor in pharmaceutical formulations and spiked human plasma using 1-dimethylaminonaphthalene-5-sulphonyl chloride reagent
Cem ÖNAL1,Şerife Evrim KEPEKÇİ TEKKELİ3
1CinnaGen Pharmaceuticals, Ataşehir, İstanbul, Turkey
2İstanbul Health and Technology University, Faculty of Pharmacy, Department of Analytical Chemistry, İstanbul Turkey
3Faculty of Pharmacy, Department of Analytical Chemistry, Bezmialem Vakıf University, Fatih, İstanbul, Turk
DOI : 10.35333/jrp.2020.229 A sensitive method is presented for the determination of the ticagrelor (TCG) in human plasma and pharmaceutical preparations using 5-(dimethylamino)naphthalene-1-sulfonyl chloride (dansyl chloride). Ticagrelor contains a secondary amino group which reacts with dansyl chloride in the presence of 0.5 M sodium bicarbonate (pH 10) to yield a highly fluorescent derivative that is measured at 445 nm after excitation at 340 nm. Different experimental parameters affecting the fluorescence intensities were carefully studied and optimized. The proposed method was also validated according to ICH guidelines. The fluorescence concentration plot was rectilinear over the range of 20.0– 200.0 ng mL−1, with a coefficient of determination of 0.9999 with a limit of detection (LOD) of 0.14 ng mL−1 and the limit of quantitation (LOQ) of 0.46 ng mL−1. The proposed method was applied successfully to the analysis of TCG in spiked human plasma and pharmaceutical dosage forms with good accuracy and precision. Keywords : Ticagrelor; antiplatelet; spectrofluorimetry; dansyl chloride; derivatization; validation; pharmaceutical preparations; plasma samples
Marmara University