Editor-in-Chief İlkay Küçükgüzel Associate Editor Esra Tatar Online ISSN 2630-6344 Publisher Marmara University Frequency Bimonthly (Six issues / year) Abbreviation J.Res.Pharm. Former Name Marmara Pharmaceutical Journal
Journal of Research in Pharmacy 2019 , Vol 23 , Issue 4
Evaluation of enzyme inhibitory and antioxidant activity of some Lamiaceae plants
Hasya Nazlı EKİN1,Didem DELİORMAN ORHAN1,İlkay ERDOĞAN ORHAN1,Nilüfer ORHAN1,Mustafa ASLAN1
1Department of Pharmacognosy, Faculty of Pharmacy, Gazi University, Yenimahalle 06330, Ankara, Turkey DOI : 10.12991/jrp.2019.184 Lamiaceae is one of the most widespread families in Turkey. The aim of this study was to determine antialzheimer, antidiabetic, antioxidant and antiobesity activities of ethanol extracts of Lamium purpureum var. purpureum, Origanum onites, Salvia sclarea, S. virgata and Thymus zygioides var. lycaonius. Acetylcholinesterase, butyrylcholinesterase, α-amylase, α-glucosidase, and pancreatic lipase inhibitory activities were tested for the determination of the activity of these extracts. Furthermore, total antioxidant, ferric-reducing antioxidant power, metal-chelating and N,N-dimethyl-p-phenylendiamine radical scavenging assays were utilized to screen antioxidant activity. Total phenolic content of the extracts were also calculated. Among the tested extracts, T. zygioides var. lycaonius aerial part extract (85.28 ± 0.89 %) showed the highest inhibitory activity against α-glucosidase. The inhibitory activities of all extracts against α-amylase were lower than 50 %. S. sclarea leaf extract indicated remarkable butyrylcholinesterase inhibition (51.76 ± 1.04 %), but all of the plant extracts were inactive against acetylcholinesterase. The Salvia species showed the highest total antioxidant activity. S. sclarea flower (1.34 ± 0.08) and leaf (1.34 ± 0.08) extracts showed the highest ferric-reducing antioxidant power activities. Our findings indicated that O. onites, S. sclarea, S. virgata, and T. zygioides var. lycaonius extracts showed valuable inhibitory activity and emerged as the sources of possible α-glucosidase inhibitors for future studies. Keywords : Lamiaceae; anticholinesterase; antidiabetic; antiobesity; antioxidant
Marmara University