Editor-in-Chief İlkay Küçükgüzel Associate Editor Esra Tatar Online ISSN 2630-6344 Publisher Marmara University Frequency Quarterly (January-April-July-October) Abbreviation J.Res.Pharm. Former Name Marmara Pharmaceutical Journal
Journal of Research in Pharmacy 2019 , Vol 23 , Issue 3
Antimicrobial activity of the crude extract, fractions and isolation of zerumbone from the rhizomes of Zingiber roseum
Mohammad Al-Amin1,Mohammad Arafat Siddiqui1,Shamima Afrin Ruma1,Nagla Mustafa Eltayeb2,Gazi Nurun Nahar Sultana3,Salizawati Muhamad Salhimi2,Chowdhury Faiz Hossain1
1Department of Pharmacy, East West University, A/2 Jahurul Islam Avenue, Jahurul Islam City, Aftabnagar, Dhaka 1212, Bangladesh
2Department of Pharmaceutical Chemistry, School of Pharmaceutical Sciences, Universiti Sains Malaysia, 11800 USM, Penang, Malaysia
3Centre for Advanced Research in Sciences (CARS), University of Dhaka, Dhaka-1000, Bangladesh
DOI : 10.12991/jrp.2019.163 The rhizome of Zingiber roseum has been used as traditional medicines for the treatment of gastric ulcer, skin diseases, and liver infections. Zerumbone is an essential sesquiterpenoid previously isolated from different species of Zingiberaceae and possesses numerous pharmacological properties. Based on previous reports, the aim of the current study was to investigate the antimicrobial activity of extract, fractions, and isolation of zerumbone from Zingiber roseum. The antimicrobial activity was determined using disc diffusion method. The serial tube dilution technique was used to determine Minimum Inhibitory Concentration (MIC) of the samples. Vacuum Liquid Chromatography (VLC) and open column chromatography were used for separation. The methanol extract exhibited maximum antimicrobial activity against Escherichia coli, Saccharomyces cerevisiae, and Aspergillus niger in both methods. VLC of the extract yielded five fractions (Fr. 1 to Fr. 5). Fr. 1 exhibited maximum antimicrobial activity compared to the other fractions. Column chromatography of Fr.1 generated 47 fractions. Fraction 1 to 9 were combined and crystallized in n-hexane to isolate zerumbone as the most abundant source (yield 6.5 % from dried MeOH extract). Zerumbone showed good activity against all tested bacteria. The results of our study might validate the ethnobotanical use of Zingiber roseum rhizomes in the treatment of infectious diseases. Moreover, Zingiber roseum could be one of the most abundant sources for the isolation of natural zerumbone to study different pharmacological studies in the field of new drug discovery. Keywords : Zingiber roseum; antimicrobial activity; MeOH extract; VLC fractions; zerumbone
Marmara University